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Currently, trying to do sim.readFromPopulationFile() raises an error if you haven't done initializeTreeSeq(). Sometimes people want to run simulations without tree sequence recording, for speed, and so do things like
c. Wrote a script to take the treesequence at time 0 and write a file in slim output
to move from a treeseq simulation to one without. However, I don't know of any reason that you shouldn't be able to load in a simulation from a tree sequence and yet not have tree sequence recording turned on? This would make it possible, for instance, to intiialize a giant simulation from an msprime simulation and run it without recording the tree sequence (for speed).
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