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Szilveszter Juhos edited this page Jul 13, 2016
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#Icing
The MinION (Oxford Nanopore) systems are relatively simple to use, and icing is HLA typing workflow based on long-range amplicon PCR that is using long reads from this sequencing platform. Hence the name: MinION rhymes with mignon that is a sort of cake in Hungary (minyon); there is usually some icing on it.
Is written in nextflow that is a DSL to make a framework around other tools used in the pipeline. To run icing you will need not only nextflow, but other tools included like BWA and canu. Once they are installed and configured correctly, the workflow should run by reading raw FASTQ files and printing out HLA genotypes.