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Hi,
You can zoom by 8x along the axis normal to the slices, which makes the resolution of your image 8 um across all dimensions (pass voxel_size=8e-6).

How to zoom: https://docs.scipy.org/doc/scipy/reference/generated/scipy.ndimage.zoom.html

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@monmon564
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@ma-sadeghi
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@jgostick
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Answer selected by ma-sadeghi
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