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Help running snow crab genome through NCBI annotation pipeline #2399

@laurahspencer

Description

@laurahspencer

Issue Category

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  • Server/Infrastructure (Raven, Klone, Gannet connectivity, performance, or storage issues)
  • Bioinformatics Tools (Tool installation, container setup, pipeline errors, workflow issues)
  • R/RStudio (Package installation, server connectivity, environment configuration)
  • Data Management (File transfers, storage, backup procedures, data organization)
  • Access Management (Account requests, permissions, authentication setup)
  • Lab Meeting Topic (Suggestions for lab meeting presentations or discussions)
  • Equipment/Hardware (Lab equipment issues, hardware problems)
  • Other (Please describe in the issue description)

Computer System Information

Please provide details about the system you're working on:

Primary system (check all that apply):

  • Raven (lab server)
  • Klone (UW HPC cluster)
  • Gannet (lab server)
  • Personal computer (Mac/Windows/Linux)
  • Other lab computer: ________________

Operating System (if known):

  • Ubuntu/Linux
  • macOS
  • Windows
  • Other: ________________

Connection method (if applicable):

  • SSH/Terminal
  • RStudio Server
  • VPN
  • Direct connection
  • Other: ________________

Issue Description

Please provide a clear description of your issue or request:

NOAA has produced a ton of snow crab / tanner crab RNASeq data that I'd like to use to improve the existing snow crab genome assembly (GCA_016584305.1). Steven mentioned the NCBI annotation pipeline is public now. I'd love some help running the pipeline on the snow crab genome, if anyone is willing!

RNASeq data is from juveniles (homogenized whole animal), and adults (separate tissues - gill, hepatopancreas, muscle), and is currently stored on a NOAA server, but I'll happily rsync RNASeq data to where it needs to go.

Error Messages

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What You've Tried

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Expected Outcome

What were you trying to accomplish or what result were you expecting?

More complete annotation for genome assembly GCA_016584305.1.

Additional Context

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