Test stability of preserved resazurin samples

[AHuffmyer]

[AHuffmyer]

Run a test in the lab to take resazurin samples out of oyster incubations and refrigerate and repeat measurements over time. The purpose is to see if we can take resazurin samples in incubations out in the field, refrigerate the plates, and then read back at UW so we don't have to transport a plate reader.

[AHuffmyer]

Are there any undergrads who could run this test in the lab? @Noah-Oz would you have time?

[kubu4]

@maddybernstein will also be able to help with this very soon.

I'll get her fully up to speed on all things resazurin and she should be able to dive in. Hopefully next week, but, if not, definitely the week after.

[Noah-Oz]

I can get it done this weekend if that's alright? But no worries if you'd rather have Maddy work on it as an introduction to resazurin!

[kubu4]

Alrighty, @maddybernstein will run an initial experiment with this starting tomorrow.

[AHuffmyer]

Looks like values are stable for 100 hours! Could we try another experiment where we compare out to 5-7 days? We can see if there is a maximum time of stability.

[AHuffmyer]

A possible experiment could be: Take 10 adults in 10 cups and stress 5 at high temp for 3-4 hours. Then take subsamples to measure fluorescence at T0, 1 hour, 24 hours, 100 hours, and 200 hours or something similar. What do you think about this?

[kubu4]

Maddy ran the first experiment last week.

Analysis is here, if you want to check it out:

https://github.com/RobertsLab/resazurin-assay-development/blob/main/scripts/fluorescence_stability/00.00-fluorescence_stability-20260528.md

[kubu4]

Gah! Never mind!

Just saw your post:

Looks like values are stable for 100 hours! Could we try another experiment where we compare out to 5-7 days? We can see if there is a maximum time of stability.

So, you obviously saw that. Haha!