Gut–Brain Axis in Alzheimer’s Disease

Code repository for metabolomics analysis and visualization in gut–brain axis research related to Alzheimer’s disease.

Description

This repository contains R scripts for:

Correlation analysis (chord diagrams, heatmaps)

Group comparisons (boxplots, volcano plots)

PCA and statistical testing on metabolomics datasets

Requirements

R (≥4.0)

Packages: circlize, ComplexHeatmap, reshape2, tidyr, dplyr, ggplot2, readxl, LMSstat, writexl

Usage

Place input data (.csv, .xlsx) in the Example data/ folder.

Open R and set the working directory inside the script.

Run the scripts (main_script.R, visualization workflow).

Outputs: correlation tables, heatmaps, chord diagrams, boxplots, volcano plots, and PCA results.