Repository of the Synthetic Biology unit - qbio
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1st semester - 5 ECTS - 40 hours in the class.
Pedagogical team: Jerome Bonnet, Guillaume Cambray, Diego Cattoni, Luca Ciandrini, Pauline Mayonove.
The majority of classes will consist of practicals in which students will explore the impact of varying regulatory elements on bacterial gene expression using a range of quantitative techniques (plate reader, cytometry) and gene expression systems (living cells and cell-free).
Students will have to present the results of their analysis during a final oral exam. They will also have to discuss an article picked from a provided list during the class.
Lectures will provide a brief overview of the field of synthetic biology, and introduce concepts that will be put into practice during this course.
| Date | Day | #hours | Time | Subject | Where |
|---|---|---|---|---|---|
| 17 November | WED | 1.5 | 9:15-10:45 | Introduction to synthetic biology (JB) | CBS |
| 17 November | WED | 1.5 | 11:00-12:30 | Genetic circuit design: programming living cells (JB) | CBS |
| 18 November | THU | 1.5 | 9:15-10:45 | DNA editing: synthesis, cloning and sequencing (GC) | CBS |
| 18 November | THU | 1.5 | 11:00-12:30 | DNA editing: synthesis, cloning and sequencing (GC) | CBS |
| 19 November | FRI | 1.5 | 9:15-10:45 | High-Throughput DNA editing to better understand translation in bacteria (GC) | CBS |
| 19 November | FRI | 1.5 | 11:00-12:30 | Short presentation by students on selected articles.(5 min/groups) Ethical Discussion | CBS |
| Date | Day | #hours | Time | Subject | |
|---|---|---|---|---|---|
| 23 November | TUE | 4 | 13:00-17:00 | Presentation of experiments, lab etiquette and good practices, colony picking and cell culture. | |
| 24 November | WED | 4 | 9:00-13:00 | Minipreps clones, send for sequencing, inoculate clones in 96 deep-well plates. | |
| 25 November | THU | 4 | 13:00-17:00 | → ALL: end point measurement on Plate reader to choose 30 clones. Group1: Plate reader group-run 30 clones growth overenight, Group2: cytometry measure the 96 clones in triplicates. | |
| 26 November | FRI | 4 | 13:00-17:00 | Group1: cytometry, Group2: Plate reader | |
| 29 November | MON | 3 | 9:00-12:00 | Group1: PCR setup for linear cell free/presentation of cell-free experimetal csv/feedback on data analysis attempts | |
| 29 November | MON | 3 | 14:00-17:00 | Group2: PCR setup for linear cell free/presentation of cell-free experimetal csv/feedback on data analysis attempts | |
| 30 November | TUE | 4 | 13:00-17:00 | Gel analysis of PCR, DNA quantification using qBit and adjustment of DNA concentrations. | |
| 1st December | WED | 4 | 13:00-17:00 | Preparing and running linear Cell Free experiement on plate reader. | |
| 2 December | THU | 4 | 13:00-17:00 | Data analysis | |
| 3 december | FRI | 4 | 13:00-17:00 | Data analysis |