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2photon_pipeline

install

install essential packages

pip install -r requirements.txt

pre-commit install

For developers, you should install pre-commit to format your code

pip install pre-commit
pre-commit install

Usage

launch GUI

python main.py

Settings

1. Select Light Field

  • None: 只进行拼接,不进行光场矫正

  • Estimate: 旧的光场估计方法,先进行高斯模糊后利用小图进行估计,可能需要手动调整bias以达到更好效果

  • NaiveEstimate(Recommended): 新的光场估计方法,利用像素合成后进行估计,加入了自适应降噪,但是对于部分花纹状噪声较多的图像,即使在降噪后上下两端还是会有一定的残留影响图像质量。因此,可以选择调整bias,通常设为bias=0.6即可,如果上下边缘还是有较明显的亮线,可以再适度调大,例如bias=1

  • BaSiC: 集成 BaSiC 算法,代码详见 https://github.com/peng-lab/BaSiCPy

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GUI tool for two-photon image stitching, including flat field correction algorithm

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  • Python 100.0%