2. Photophysics

Quantification of SMLM data often requires calibration measurements to characterize the photoswitching of a certain dye in your imaging system (buffer, microscope, etc.).

An example would be the determination of the localization precision (sigma). Here, single labels, such as a conjugated antibody are spotted on a cleaned glass surface and then imaged under experimental conditions.

The scripts in the folder Photophysics help to analyze such datasets.