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Welcome to the SMLM Tutorial wiki!

Single-molecule localization microscopy (SMLM) relies on the temporally separated emission of individual molecules. While the acquired image is still diffraction limited, due to their sparsity, each molecule can be localized at nanometer precision and their coordinates accumulated to form a super-resolved image. But the power of SMLM goes far beyond generating images. Computational analysis tools allow extracting the spatial organization, colocalization, clustering and dynamics of individual molecules, which has transformed the study of nanoscale biology over the past decade.