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2D-Map Viewer

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@diogobor diogobor released this 03 Mar 22:08
· 1 commit to main since this release
9675d45
  • (new) 2D-Map Viewer: Scout has introduced a new circular visualisation tool designed to display identified protein-protein interactions (PPIs) in an interactive and intuitive layout. The top 50 highest-scoring proteins are rendered arranged around a ring, with cross-links drawn between them to represent interactions. More information in section 2.3.
  • (new) Statistics → PPM Distribution: Scout now is able to view the PPM distribution at the MS1 level. In addition, the graph can be saved as a PNG or SVG file. To do this, right-click on the graph and select "Save".
  • (new) Search parameters → Cleavable Reagent: fields for α and β target residues now exist.
  • (new) Perform Bruker® pre-processing: Scout is able to perform preprocessing analyses on timsTOF data. However, if the file has already been preprocessed by Bruker® DataAnalysis software, Scout will be able to read the generated mgf file. To do this, uncheck this option.
  • (new) mzIdentML export in CLI mode: Scout can now export its results to the mzIdentML 1.2 or 1.3 format. To do this, type the following command:
    • scout.exe -mzid -v <1.2 or 1.3> -i scout_file -raws path_raw_files -o mzid_output_file (for Windows)
    • ./run_scout.sh -mzid -v <1.2 or 1.3> -i scout_file -raws path_raw_files -o mzid_output_file (for Linux or macOS)
  • bug fixed: Protein sequence was palindromically duplicated in mzIdentML → this bug has been fixed.
  • bug fixed: The Bruker® .d file was not working with the parser → this bug has been fixed.
  • bug fixed: Unfiltered CSMs were not working in CLI mode → this bug has been fixed. The corrected command is:
    • scout.exe -unfiltered_csm -i scout_file -o path_to_output_file (for Windows)
    • ./run_scout.sh -unfiltered_csm -i scout_file -o path_to_output_file (for Linux or macOS)
  • minor modification: Confirm and Cancel buttons have been added to the Search Parameters, Advanced Search Parameters, and Post-Processing Parameters windows.
  • minor modification: Scout does not recognize Python installations performed through the Windows Store. Only installations via python.org or Anaconda are recognized.
  • minor modification: Scout_search_params.json example file has been updated.