Intronserter back-translates an amino acid sequence into its codon-optimized DNA variant, removing cut sites by synonymous codon replacement and distributing regulatory introns into the DNA sequence following the systematic approach outlined for the green alga Chlamydomonas reinhardtii in Baier et al. 2018 (Baier, T., Wichmann, J., Kruse, O., Lauersen, K.J., "Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii", Nucleic Acids Research (2018), doi.
An user friendly online version is available at BiBiServ.
Intronserter is published as:
Jaeger, D., Baier, T. and Lauersen, K.J., "Intronserter, an advanced online tool for design of intron containing transgenes", Algal Research (2019), doi.