🧪 CellSplit | Host Cell Lab Suite

Precision cell passaging. Zero friction.

CellSplit is a minimalist, professional-grade tool designed to streamline mammalian cell culture maintenance. It bridges the gap between Neubauer chamber counting and inoculum calculation in a single, high-contrast interface.

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🧬 Scientific Fundamentals

CellSplit automates the critical calculations required for bioprocess scaling and cell line maintenance (optimized for CHO cells).

1. Viable Cell Concentration

The app calculates the density of viable cells per milliliter based on your count and dilution factor:

$$\text{cell/mL} = \left( \frac{\text{Live Cells counted}}{\text{Squares counted}} \right) \times \text{Dilution Factor} \times 10,000$$

2. Viability (%)

Determines the health of the culture using dye exclusion (e.g., Trypan Blue):

$$\text{Viability (%)} = \left( \frac{\text{Live Cells}}{\text{Live Cells} + \text{Dead Cells}} \right) \times 100$$

3. Inoculum Volume (Passaging)

Applies the conservation of mass principle ($C_1 V_1 = C_2 V_2$) to determine the exact volume needed for a new flask:

$$V_1 = \frac{C_2 \times V_2}{C_1}$$

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🔍 Equipment Specifications

CellSplit's algorithms assume the use of a standard Neubauer counting chamber (or hemocytometer) with the following precision dimensions:

• Chamber Depth: $0.1$ mm.
• Smallest Square Area: $0.0025 \text{ mm}^2$ ($0.05$ mm side length).
• Conversion Factor: The $10,000$ ($10^4$) factor is derived from the volume of one large square ($1 \text{ mm}^2 \text{ area} \times 0.1 \text{ mm} \text{ depth} = 0.1 \text{ mm}^3$), which is equivalent to $10^{-4} \text{ mL}$.

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⚡ Features

• Automated Data Link: Results from the counting module are automatically transferred to the passaging module.
• Range Diagnostics: Visual alerts if your count is outside the optimal statistical range (100–500 cells in 10 squares).
• PWA Ready: Install it on Android or iOS for offline use inside the biosafety cabinet.
• Dark Mode UX: Designed for high visibility in low-light microscope rooms.

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❓ FAQ (Frequently Asked Questions)

Q: Why does the app use the $10,000$ factor? A: Since one large square on a Neubauer chamber represents a volume of $0.1 \text{ mm}^3$, multiplying by $10,000$ converts the average count per square into cells per $1 \text{ mL}$ ($1,000 \text{ mm}^3$).

Q: Can I use this for non-CHO cells? A: Yes. While developed for CHO cell bioprocessing, the math is universal for any mammalian cell line counted via hemocytometer.

Q: Does it work without internet? A: Yes. Once installed as a PWA, the Service Worker caches the logic for offline lab use.

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Installation / PWA (optional)

MolarPrep can be installed as a Progressive Web App (PWA) on supported browsers.

Desktop / Android

• Open the live app
• Click the Install button (if shown), or use browser install prompt

iPhone / iPad (Safari)

• Open the live app
• Tap Share
• Select Add to Home Screen

Once installed, it can work offline after the necessary files are cached.

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👨‍🔬 Author

Emiliano Balderas Biotechnology Engineer | PhD Student in Biochemistry Instituto de Biotecnología (IBt) - UNAM.

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Part of the Host Cell Suite

MolarPrep is part of Host Cell, a suite of practical tools for bioprocessing and lab workflows.