For Data processing In GSM1827405 author wrote that "Overlapping reads were merged using SeqPrep and split in half with a Awk routine. Paired-ends with one read shorter than 30 bp were discarded."
I run below codein my linux:
seqprep -f SRR2017741_1.fastq.gz -r SRR2017741_2.fastq.gz -1 SRR2017741_1_seqprep.fastq.gz -2 SRR2017741_2_seqprep.fastq.gz
and I get below result:
Pairs Processed: 37404976
Pairs Merged: 0
Pairs With Adapters: 75653
Pairs Discarded: 28621
CPU Time Used (Minutes): 55.881797
I don't know why Pairs Merged is 0? I appreciate if you share your comment with me for finding my problem and completing my seqprep code for this sample.
For Data processing In GSM1827405 author wrote that "Overlapping reads were merged using SeqPrep and split in half with a Awk routine. Paired-ends with one read shorter than 30 bp were discarded."
I run below codein my linux:
seqprep -f SRR2017741_1.fastq.gz -r SRR2017741_2.fastq.gz -1 SRR2017741_1_seqprep.fastq.gz -2 SRR2017741_2_seqprep.fastq.gzand I get below result:
I don't know why Pairs Merged is 0? I appreciate if you share your comment with me for finding my problem and completing my
seqprepcode for this sample.