v0.10.1

Picasso 0.10.1 is here

Changelog

Localize

• Fixed Gauss-fitting error when spot's sum is zero (zero division error)

Render

• Faster rendering through improvements for all blur methods and multi-level spatial indexing for quick zoomed-in rendering
• Multichannel rendering supports colormaps, not only a single RGB color
• Anisotripic DBSCAN (with faster implementation) DOI: 10.1021/acs.jpcb.4c02030
• Faster cluster centers calculation for SMLM clusterer, DBSCAN and HDBSCAN + lpz is saved if applicable
• Load FOV keeps the aspect ratio of the input .txt file
• Files dialog resizing fixed
• Show 3D clustering widgets only when 3D data is loaded
• Fixed linking saving lpz
• Fixed high-resolution display of mask in Render (#666)
• Removed render property cache since it did not provide any significant speed improvement

SPINNA

• Comparing models uses fitting modes (Bayesian, etc) and has a cleaner progress dialog
• Convenient fitting of LE
• Area/volume button removed (deduced automatically from densities and number of molecules in the exp. data)
• Show 3D masking widgets only if 3D mask is selected
• Batch analysis for LE fitting
• Batch analysis does not require area input (if found in metadata)
• Batch analysis has clear instructions on what columns are required via CLI and the docs update
• Fixed NND plot reindexing after fitting (#665)

Filter

• Efficient Filter: much lower RAM usage + faster filtering by histogram selection (1D/2D), especially for very large datasets

Others

• Expanded the scope of the sample notebooks
• Improved docstrings for 3D SMLM clusterer
• Flake8 clean-up
• Installers are distributed with readme.txt files (previously .rst)
• Minor changes to documentation
• Fixed subcluster check plot when one of the two populations is empty (#667)
• Fixed 2D fitting with console printout and no multiprocessing

Notes

⚠️ If installed in Program Files, Render and Localize may not be available for non-administrator users. Therefore, we recommend installing Picasso outside of Program Files. The current default location is C:\Picasso.
⚠️ When using Windows installer, camera config file needs to be moved to C:\Picasso\_internal\picasso. Previously under C:\Picasso\picasso.
⚠️ Windows Safety features and Windows Defender may ask multiple times for permission during the installation and download.
⚠️ If your macOS installer does not open due to potential malware, click on the "?" button and follow the instructions to open Picasso anyway. This needs to be repeated for both the main "picasso" app as well as the individual modules.