v0.9.5

Picasso 0.9.5 is here

Changelog

Important updates:

• Algorithm for molecular mapping introduced (G5M), see documentation. DOI: 10.1038/s41467-026-70198-5.
• Localize outputs axial localization precision for astigmatic imaging in 3D, DOI: 10.1038/s41467-026-70198-5.
• Localize GUI allows the user to select which localization columns to save when saving localizations. See the new dialog in the File -> Select columns to save
• Localize accepts frame bounds to analyze only a subset of frames
• Config file accepts z calibration .yaml paths so that they can be automatically loaded when changing between cameras
• Render by property (GUI) shows histogram of the selected property
• Filter GUI has a new plot to test for subclustering based on the number of events per molecule (column n_events); see documentation for details

Small improvements:

• Picasso applies constrained layout to all matplotlib figures
• SPINNA uses FigureCanvas instead of QSvgRenderer for displaying NND plots and mask legend
• SPINNA default mask blur set to 500 nm
• 3D animation saves metadata
• Some improvements in how DataFrames are handled (Filter, change from .values to .to_numpy())

Bug fixes:

• Render GUI takes camera pixel size using lib.get_from_metadata (#602)
• Render by property is switched off if more than one channel is loaded
• Render 3D scale bar manual adjustment fixed
• Render 3D screenshot .yaml fixed
• .tif IO bug fix related to the numpy deprecation of arr.newbyteorder (#603)
• Clarify GPU fit installation instructions and remove version printing (#604)
• SPINNA fixed loading of the proportion spin boxes after rerunning SPINNA, such that they add up to 100% again

Notes

‼️ If installed in Program Files, Render and Localize may not be available for non-administrator users. Therefore, we recommend installing Picasso outside of Program Files. The current default location is C:\Picasso.
⚠️ Windows Safety features and Windows Defender may ask multiple times for permission during the installation and download.