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Release notes v0.16.25
Kenji Fukushima edited this page Jun 8, 2026
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These notes summarize the kfdevel line prepared for merge into master.
- AMALGKIT is now Python-only for the main pipeline. R scripts and R smoke tests have been removed.
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amalgkit confighas been removed. Selection rules now live in oneselect_rules.tsvfile. -
amalgkit curatehas been replaced by the split workflowwsfilter -> csfilter -> finalize. -
amalgkit cscahas been replaced by current cross-species filtering and finalization outputs. -
amalgkit rerunwas added to rerun failed targets fromsanity_report.json.
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amalgkit dataset --rule_set base|test|plantae|vertebrateexports bundledselect_rules.tsv. -
amalgkit dataset --name initcreates an empty workspace scaffold. -
amalgkit selectsupports species-wise batch mode and writes summary, queue, and manifest tables.
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getfastqsupports NCBI, AWS, GCP, ENA, and DDBJ provider fallbacks. - Shared download locks and provider concurrency caps help large parallel runs.
- Optional MMseqs2 rRNA and contaminant filters can be ordered with
--filter_order. -
quantcan auto-select kallisto for short-read data and oarfish for long-read data. - Quant index builds use shared locks to avoid duplicate builds under array jobs.
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wsfilterperforms within-species filtering and writes filtered metadata plus PDFs. -
csfilterperforms cross-species filtering with BUSCO or orthogroup inputs. -
finalizeexports final per-species expression tables and supports Python backends forno,sva,ruvseq,combatseq, andlatent_glm.
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sanitychecks getfastq, index, quant, merge, busco, and finalize outputs. -
rerunresolves missing or failed targets from sanity reports and writesrerun_manifest.json.
| Old command | Current replacement |
|---|---|
amalgkit config |
amalgkit dataset --rule_set ... and select_rules.tsv
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amalgkit curate |
amalgkit wsfilter, amalgkit csfilter, amalgkit finalize
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amalgkit csca |
amalgkit csfilter and amalgkit finalize
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