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Core

Language: R

Input: TXT

Output: PREFIX

Tested with: PluMA 1.1, R 4.0.0

Dependencies: phyloseq 1.32.0, ape 5.4, psadd 0.1.2, ggplot2 3.3.1, microbiome 1.10.0

PluMA plugin that outputs various statistics on core communities of taxa in a set of samples.

The following are specified in the input TXT file, as tab-delimited keyword-value pairs.

otufile: OTU abundances (CSV) mapping: Mapping table (CSV) tree: Phylogenetic tree (CSV) column: (STRING) detection: Minimum relative abundance to be counted at all (REAL) prevalence: Minimum relative abundance to be counted as core

The output prefix will be used for three files: prefix.members.csv: Core taxa prefix.abundindex.csv: Percentage of each sample that is a core community prefix.coverage.csv: Amount of core taxa sequences in each sample

Format of the otufile CSV:

  • Rows are OTUs, columns are samples
  • Entry (i,j) is the abundance of OTU in sample j. Can be absolute or relative.

Format of the mapping CSV:

  • Rows are samples, columns are metadata
  • One single header row, i.e: sample,subject,visit

Format of the tree CSV;

  • Rows are OTUs, columns are classifications
  • One single header row, i.e. OTU,Kingdom,Phylum,Class,Order,Family,Genus,Species

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Statistics on core taxa communities

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