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Dev metagenomics low biomass bn#195

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bnovak32 merged 13 commits intonasa:DEV_Metagenomics_low_biomassfrom
bnovak32:DEV_Metagenomics_low_biomass_BN
Mar 19, 2026
Merged

Dev metagenomics low biomass bn#195
bnovak32 merged 13 commits intonasa:DEV_Metagenomics_low_biomassfrom
bnovak32:DEV_Metagenomics_low_biomass_BN

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@bnovak32 bnovak32 commented Mar 4, 2026

Updates to low biomass pipeline docs

bnovak32 added 13 commits March 3, 2026 17:48
- fixed spelling/typos across both documents
- Illumina updates
  - removed remove human reads step and added reference to remove human
    reads pipeline
  - renumbered steps and fixed internal links
- add missing suffixes
- fix incorrect suffixes
- remove unneeded file suffixes (only needed on published files)
- remove unused samtools index step in Assembly-based processing
- changed csv to tsv
- updated feature_decontam method to create correctly named files
- removed humann from software table in long read pipeline
- updated output naming for humann outputs from path- to pathway-
- fix broken links
- add missing filtering steps in Assembly-based processing
- synchronize assembly-based downstream processing with workflow draft
- standardize naming of output files in Assembly-based downstream
  processing
- Added top50 heatmap pngs generation
- Fixed defintions for heatmaps and contig-level output
- added note about decontaminated plots and species tables only being
  present when 1 or more contaminants found
- fixed assay suffixes in GL-DPPD-7116
- reorganize R code
- update thresholds to match current implementation
- update kraken2 build commands to use k2 wrapper and add pointers to
  RemoveHumanReads pipeline
- refactor R code in long-read pipeline to match short-read and workflow
  implementation
- update thresholds and defaults to match current implementation
@bnovak32 bnovak32 merged commit 7366989 into nasa:DEV_Metagenomics_low_biomass Mar 19, 2026
@bnovak32 bnovak32 deleted the DEV_Metagenomics_low_biomass_BN branch April 15, 2026 16:18
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