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21 changes: 21 additions & 0 deletions LICENSE
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@@ -0,0 +1,21 @@
MIT License

Copyright (c) 2020 Shruti Sharma

Permission is hereby granted, free of charge, to any person obtaining a copy
of this software and associated documentation files (the "Software"), to deal
in the Software without restriction, including without limitation the rights
to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
copies of the Software, and to permit persons to whom the Software is
furnished to do so, subject to the following conditions:

The above copyright notice and this permission notice shall be included in all
copies or substantial portions of the Software.

THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
SOFTWARE.
25 changes: 23 additions & 2 deletions README.md
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# gunzip_fqgz_process

The nextflow wrapper for the `gunzip` process.
## This nextflow wrapper helps to implement `gunzip` process of a `fastq` pipeline that includes:

https://github.com/nextflow-hub/trimmed_gunzip_fqgz
1. Define a default parameter `params.trimmed=true`
2. Check if the `inputRawFilePattern` is trimmed or untrimmed
3. The `trimmed` file has `p` in the file pattern whereas untrimmed file does not have `p` in the file pattern
4. Based on the file pattern the input parameter of the script is defined
5. Execute the script to gunzip the input file using `gzip –dc` command and stores the output into the `publishDir`


## Execute the script by using the following command:

```nextflow run main.nf```

You will see the execution of a `gzip` process.

## Execute it again adding the -resume option as shown below:

```nextflow run main.nf -resume```

The -resume option skips the execution of any step that has been processed in a previous execution.

## Adding a publishDir directive to the gzip process to store the process results into a directory of your choice.

In this script, it stores the result files locally into `results/gzip` directory.
22 changes: 16 additions & 6 deletions main.nf
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@@ -1,13 +1,22 @@
#!/usr/bin/env nextflow


/*
#######################
Code documentation
#######################
1. Define a default parameter `params.trimmed=true`
2. Check if the `inputRawFilePattern` is trimmed or untrimmed
3. The `trimmed` file has `p` in the file pattern whereas untrimmed file does not have `p` in the file pattern
4. Based on the file pattern the input parameter of the script is defined
5. Execute the script to gunzip the input file using `gzip –dc` command and stores the output into the `publishDir`
*/

/*
################
params
################
*/
//NOTE: default parameter

params.trimmed=true


Expand All @@ -20,7 +29,7 @@ NEXTFLOW Global Config

inputUntrimmedRawFilePattern = "./*_{R1,R2}.fastq.gz"

//NOTE: This pipeline is starts with `trimmed` because of the `p` in the file pattern.

inputTrimmedRawFilePattern = "./*_{R1,R2}.p.fastq.gz"

inputRawFilePattern = params.trimmed ? inputTrimmedRawFilePattern : inputUntrimmedRawFilePattern
Expand Down Expand Up @@ -49,14 +58,15 @@ process gzip {
script:
outputExtension = params.trimmed ? '.p.fastq' : '.fastq'

// rename the output files
// G04880_R1.p.fastq.gz > G04880_R1.p.fastq
/* rename the output files
XYZ.p.fastq.gz XYZ.p.fastq
*/
genome_1_fq = read_1_gz.name.split("\\.")[0] + outputExtension
genome_2_fq = read_2_gz.name.split("\\.")[0] + outputExtension

"""
gzip -dc ${read_1_gz} > ${genome_1_fq}
gzip -dc ${read_2_gz} > ${genome_2_fq}
"""
//gzip -dc G04880_R1.p.fastq.gz > G04880_R1.p.fastq
//gzip -dc XYZ_R1.p.fastq.gz > XYZ_R1.p.fastq
}