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here is the outputs when executing rMSA.pl
creating /tmp/root/rMSA_23925_928759702
>/home/rMSA/fasta/3/3.fasta length=15
AAAATCAAAAAGAAG
using 4 cpu
cp /home/rMSA/fasta/3/3.dbn /tmp/root/rMSA_23925_928759702/RNAfold.dbn
==== rfam pre-screening ====
wc: /tmp/root/rMSA_23925_928759702/rfam*.db: No such file or directory
wc: /tmp/root/rMSA_23925_928759702/db0: No such file or directory
0 total
==== blastn-short pre-screening ====
/home/rMSA/bin/blastn -num_threads 4 -query /tmp/root/rMSA_23925_928759702/seq.fasta -strand plus -db /home/rMSA/database/rnacentral.fasta -out /tmp/root/rMSA_23925_928759702/blastn0.tab -task blastn-short -max_target_seqs 50000 -outfmt '6 saccver sstart send evalue bitscore nident staxids'
cut -f1 /tmp/root/rMSA_23925_928759702/blastn0.tab|sort|uniq > /tmp/root/rMSA_23925_928759702/blastn0.list
split -l 5000 /tmp/root/rMSA_23925_928759702/blastn0.list /tmp/root/rMSA_23925_928759702/blastn0.list.split.
cat /tmp/root/rMSA_23925_928759702/blastn0.trim.split.* | /home/rMSA/bin/fasta2pfam - | sort -u -k2 | /home/rMSA/bin/pfam2fasta - > /tmp/root/rMSA_23925_928759702/blastn0.db
cat: /tmp/root/rMSA_23925_928759702/blastn0.trim.split.*: No such file or directory
rm /tmp/root/rMSA_23925_928759702/blastn0.trim.split.*
rm: cannot remove ‘/tmp/root/rMSA_23925_928759702/blastn0.trim.split.*’: No such file or directory
/home/rMSA/bin/blastn -num_threads 4 -query /tmp/root/rMSA_23925_928759702/seq.fasta -strand both -db /home/rMSA/database/nt -out /tmp/root/rMSA_23925_928759702/blastn1.tab -task blastn-short -max_target_seqs 50000 -outfmt '6 saccver sstart send evalue bitscore nident staxids'
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