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general fixes 11-2025 #3489
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general fixes 11-2025 #3489
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| Original file line number | Diff line number | Diff line change |
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@@ -72,7 +72,7 @@ Shotgun sequencing | |
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| Qiita currently has one active shotgun metagenomics data analysis pipeline: a per sample, paired-end | ||
| bowtie2 alignment step with Woltka classification using either the WoLr2 (default) or RS210 databases. | ||
| bowtie2 alignment step with Woltka classification using either the WoLr2 (default) or RS225 databases. | ||
| Below you will find more information about each of these options. | ||
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| .. note:: | ||
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@@ -197,6 +197,23 @@ Note that some of these are legacy option but not available for new processing. | |
| - Genera: 6,811 | ||
| - Species: 12,258 | ||
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| #. RS225: Collection of reference microbial genomes sampled from the NCBI | ||
|
Collaborator
There was a problem hiding this comment. Choose a reason for hiding this commentThe reason will be displayed to describe this comment to others. Learn more. Great! |
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| RefSeq genome database, as of 2024-08-01. This time point corresponds to | ||
| RefSeq release 225. RS225 contains 40,987 genomes from NCBI RefSeq and | ||
| 11,771 genomes from external sources. The total number of genomes, 52,758, | ||
| represents an 78% increase from the previous version of the database. | ||
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| - Total number of genomes: 52,758 | ||
| - Total length of genomes (after adding linkers): 170,326,480,530 bp | ||
| - Number of genomes by category: | ||
| - Archaea: 870 | ||
| - Bacteria: 32,894 | ||
| - Fungi: 610 | ||
| - Protozoa: 93 | ||
| - Viral: 18,279 | ||
| - SynDNA Constructs: 12 | ||
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| #. RS210: Collection of reference microbial genomes sampled from the NCBI RefSeq | ||
| genome database, as of 2022-01-01. This time point corresponds to RefSeq | ||
| release 210. | ||
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@@ -212,6 +229,7 @@ Note that some of these are legacy option but not available for new processing. | |
| - Protozoa: 93 | ||
| - Viral: 7,493 | ||
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| #. WoLr1 ("Web of Life" release 1): An even representation of microbial diversity, selected using an prototype | ||
| selection algorithm based on the MinHash distance matrix among all non-redundant bacterial and archaeal genomes | ||
| from NCBI (RefSeq and GenBank, complete and draft), plus several genome quality control criteria. A | ||
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@@ -236,6 +254,7 @@ Note that some of these are legacy option but not available for new processing. | |
| - Strains: 89 | ||
| - Note: Nucleotide sequences per genome were concatenated with a linker of 20 "N"s. | ||
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| #. Rep200: NCBI representative and reference microbial genomes, corresponding to RefSeq release 200 (2020-05-14) | ||
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| - Genomes: 11,955 | ||
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@@ -249,6 +268,7 @@ Note that some of these are legacy option but not available for new processing. | |
| - Protozoa: 88 | ||
| - Viral: 48 | ||
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| #. Rep94: NCBI representative and reference microbial genomes, corresponding to RefSeq release 94. | ||
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| - Domains: Bacteria, Archaea | ||
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@@ -266,6 +286,7 @@ Note that some of these are legacy option but not available for new processing. | |
| - Species: 5,636 | ||
| - Strains: 84 | ||
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| #. Rep82: NCBI representative and reference microbial genomes, corresponding to RefSeq release 82. | ||
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| - Not available anymore for new processing | ||
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@@ -284,6 +305,7 @@ Note that some of these are legacy option but not available for new processing. | |
| - Species: 11,852 | ||
| - Strains: 4,263 | ||
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| Metatranscriptome processing | ||
| ---------------------------- | ||
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This seems a bit random?
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Well, it's helpful when debugging; I don't even remember when but I had it in my notes to improve.