hisat2-build RAM 不足

[shenweiyan]

在 96G RAM 的节点跑 T2T-CHM13v2.0 的 HISAT2 index 时候，发现任务居然被系统 Killed 掉了。

排查一下才发现是因为 RAM 内存不够！

Note: If you use --snp, --ss, and/or --exon, hisat2-build will need about 200 GB RAM for the human genome size as index building involves a graph construction. Otherwise, you will be able to build an index on your desktop with 8GB RAM.

From hisat2-build manual with usage examples | BioQueue Encyclopedia