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gffread / salmon / hisat2 not found in Snakemake shell rules. The make_transcriptome, salmon_index, and hisat2_index rules now explicitly prepend ${MAMBA_ROOT_PREFIX}/envs/bulkseq/bin to PATH at the start of their shell commands. Snakemake's subprocess environment does not reliably inherit the micromamba-activated PATH in all configurations; this fixes the "gffread is not installed" error even when the binary is present in the env.