Overview

Ribo-seq analysis for SLFN14 manuscript

Requirements

All the environment of the analysis steps were developed as docker images.

Contents

Preprocessing

preprocessing/

Adapter removal, rRNA mapping, and mRNA mapping of the reads.

All the steps were done in a docker image defined in preprocessing/dockerfile.

A custom reference transcriptome was generated by the code in preprocessing/ref.

Ribo-seq analysis

riboseq/

Generating count data and making figures.

All the steps were done in a remote container of VScode defined in riboseq/devcontainer

The figures were made by a custom python code under riboseq/.

Contact

Saori Uematsu: su64@cornell.edu, saorid6e9p6p9@gmail.com