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VaxPress

Introduction

VaxPress is a powerful codon optimization tool designed specifically for mRNA vaccine development. It uses various biological factors such as iCodon stability, Codon Adaptation Index (CAI), U count minimization, RNA folding, GC ratio, and tandem repeats to optimize codon usage, aiming to enhance mRNA stability, translation efficiency, and immunogenicity.

Installation

pip

You can install VaxPress via pip:

pip install vaxpress

conda

conda install -c conda-forge -c bioconda -c changlabsnu vaxpress

Singularity

First, you will need to install Singularity CE. Once Singularity is installed, you can download the VaxPress image from our GitHub repository and run using the following command:

singularity vaxpress.sif ...

Usage

vaxpress -i INPUT -o OUTPUT

For more detailed usage instructions including all the available options, please refer to the usage guide.

Input/Output Options

These options include input fasta file, whether input is a protein sequence, output directory, etc.

Execution Options

These options include preset values, number of processes, random seed, target species, etc.

Optimization Options

These options include number of iterations, offsprings per iteration, survivors per iteration, initial mutation rate, etc.

Fitness Options

These options include scoring weights for iCodon predicted stability, CAI, bicodon-weight, U count minimizer, RNA folding, GC ratio, tandem repeats, etc.

License

This project is licensed under the MIT License.

Contact

If you have any questions or feedback, please feel free to contact us.

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Codon Optimizer for mRNA Vaccine Design

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