ThunderSTORM Origami Analyzer v0.0.4

Detection & analysis (app + Fiji plugin)

• Generalised DNA-origami detection from fixed triplets to arbitrary N-spot collinear structures; "triplet" renamed to "structure" throughout.
• Merged the separate spacing and NND-target inputs into one "Spacing / NND" value (+ tolerance); the NND-target band is derived from it.
• Optics reworked into clearly labelled Camera pixel size + Objective magnification + Visualization magnification. Effective pixel = camera ÷ objective; the SR render bin is auto-derived (= effective ÷ visualization), shown live and overridable per dataset — the bin label now also reflects a manual edit.
• NND distribution plot x-axis now stretches to the target spacing ± tolerance.
• Scale bars on the super-resolution, structure-zoom and carousel images are anchored to spacing + tolerance (a clean round multiple on large fields), with correct nm/µm units.
• New "Real calibration" toggle (default off): intensity is labelled "a.u." unless enabled, then "photon" (echoing the protocol's offset / photons2ADU / EM-gain, with an inconsistency warning). Values are never recomputed — labelling only.

Desktop app

• Faster CSV browsing (header-only read).
• Per-dataset QC auto-fill: max sigma / max uncertainty from the data's 95th percentile.
• The dataset list no longer jumps to the top while editing a field.

Fiji plugin (1.0.2)

• Window now uses the Fiji/ImageJ logo.
• Much faster CSV loading (buffered streaming read) — fixes the apparent hang on large files.

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Bundled Fiji/ImageJ plugin: ThunderSTORMAnalyzer-Fiji-plugin.jar (v1.0.2)

ThunderSTORM Origami Analyzer v0.0.3

• matplotlib>=3.8,<3.11
• wrapped histograms in try/except

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Bundled Fiji/ImageJ plugin: ThunderSTORMAnalyzer-Fiji-plugin.jar (v1.0.1)

ThunderSTORM Origami Analyzer v0.0.2

• splash startup (splash shows before heavy libraries load).
• Fiji plugin: min-blink-cycles / min-active-frames now affect detection.
• Check for updates

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Bundled Fiji/ImageJ plugin: ThunderSTORMAnalyzer-Fiji-plugin.jar (v1.0.1)