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Single-cell insights into the alleviation of diabetic nephropathy by Rosmarinic acid

Collection of R codes and horse perl scripts to perfrom analysis and data visualization in research article "Single-cell transcriptomics reveals the ameliorative effect of Rosmarinic acid on diabetic nephropathy-induced kidney injury by modulating oxidative stress and inflammation".

Authors Junhui Chen (chenjunhui)
Email chenjhbio@163.com
License GPLv3

Citations

If you used the codes or reference part of the code in your research, please kindly cited following paper: Chen et al. ABSP (2022) Single-cell transcriptomics reveals the ameliorative effect of Rosmarinic acid on diabetic nephropathy-induced kidney injury by modulating oxidative stress and inflammation

Dependece packages

Following are a list of R packages that are used by the analysis pipeline. Before implement the codes, please comfirmed the packages have been installed on your R platform.

  • Seaurat version 4.1.0
  • DoubletFinder version 2.02
  • clusterProfiler version 4.0.0
  • SCP version: 0.5.6
  • ggplot2 version: 3.3.5

The other R packages such as dplyr, should depend on R environment variable space to install the responding packages as following commands:

install.packages(dplyr) 
or
BiocManager::install(dplyr)

Installation

Just download the codes and implement in R platform under linux or window system. For packages installation, mainly intallated through conda or build-in funciont of R install.packages()

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Code for research article of RA treatment on DKD

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