v0.1.0 Technical Preview

Changelog

All notable changes to this project will be documented in this file.

The format is based on Keep a Changelog,
and this project adheres to Semantic Versioning.

[0.1.0] - 2025-11-27

Added

• Core Framework:
  • Modular 3-layer architecture (CLI, Workflows, Core).
  • Unified YAML configuration system.
  • Robust logging and progress tracking.
• PCR Workflow:
  • Automated primer design using Primer3.
  • Comprehensive QC (Tm, GC, Hairpin, Homodimer, Heterodimer).
  • JSON and Markdown report generation.
• qPCR Workflow:
  • TaqMan® probe design support.
  • Primer-Probe compatibility checks.
  • Efficiency estimation logic.
• API:
  • Programmatic access via primerlab.api.public.
  • Functions: design_pcr_primers, design_qpcr_assays.
• Testing:
  • Full pytest suite covering PCR, qPCR, and API.
  • CI/CD integration via GitHub Actions.

Fixed

• Critical bug in reverse primer coordinate calculation (Primer3 3' index vs 5' start).
• QC silent pass bug when ViennaRNA is missing (now raises explicit warnings).
• Timeout handling for stuck Primer3 processes.

Changed

• Switched to pyproject.toml for modern packaging (PEP 621).
• Updated documentation structure for long-term roadmap.