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Version-1.02.6

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@nadiadavidson nadiadavidson released this 30 Mar 03:57
· 6 commits to main since this release

This release fixes minor bugs and improves support when multiple barcodes need to be demultiplexed from each read

It now splits the former -i option into two:
-l true/false Replace read ID with barcodes+UMI (default: true)
-r true/false Remove search strings including flanking sequence and split read if multiple barcodes found (default: true).

This allow the barcode region to be left in the read sequence, whist adding identified barcodes into the read header (-l true -r false), meaning the entire region can be used to anchor the search for each barcode that it demultiplexed.

See the documentation for an example of how to run flexiplex to demultiplex multiplex barcode within each read.