v0.1.0

Initial release of PIE-scope GUI.

Dependencies

Hardware requirements

• FIB/SEM microscope (a commercial product by ThermoFisher FEI)
• Basler detector (https://www.baslerweb.com)
• Toptica lasers (for the fluorescence microscope)
• SMARACT stage (controlling the fluorescence objective lens position)

Software requirements

• Python 3.6
• Autoscript software (a commercial product by ThermoFisher FEI)
• The Basler Pylon Software Suite (https://www.baslerweb.com/en/products/software/basler-pylon-camera-software-suite/)
• The Basler pypylon python package (https://github.com/basler/pypylon)
• The piescope python package containing back end functionality for PIE-scope operations (https://github.com/DeMarcoLab/piescope/releases)
• Python libraries (these will be installed by default for you):
  • click
  • matplotlib>=3.0.0
  • pyqt5
  • qimage2ndarray
  • scikit-image>=0.15.0
  • scipy

To install piescope, download the wheel file (.whl) from the assets below and follow the installation instructions.