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createArrowFiles has encountered an error #2287

@Tc940417

Description

@Tc940417

I have a trouble about createArrowFiles ,there is my code and log. thanks!

Attach your log file

ArchR-createArrows-16c816882c00-Date-2025-03-31_Time-12-16-09.425782.log

Describe the bug
createArrowFiles has encountered an error, checking if any ArrowFiles completed..

Screenshots

library(parallel)
library(ArchR)
library(org.At.tair.db)
library(GenomicRanges)
library(TxDb.Athaliana.TAIR.10)
library(BSgenome.Athaliana.TAIR.10)
TxDb <- TxDb.Athaliana.TAIR.10
OrgDb <- org.At.tair.db

addArchRChrPrefix(chrPrefix = FALSE)
ArchR is now disabling the requirement of chromosome prefix = 'chr'
geneAnno <- createGeneAnnotation(TxDb=TxDb, OrgDb=OrgDb, annoStyle = "TAIR")
Getting Genes..
Determined Annotation Style = TAIR
Getting Exons..
Getting TSS..
genomeAnno <- createGenomeAnnotation(genome = BSgenome.Athaliana.TAIR.10)
Getting genome..
Attempting to infer chromSizes..
Attempting to infer blacklist..
Blacklist not downloaded! Continuing without, be careful for downstream biases..

input.file.list <- c("D:/sn-CUT&TAG/2_fragments/ara.frag/ara9/Ara9.fragments.tsv.gz",

  •                  "D:/sn-CUT&TAG/2_fragments/ara.frag/ara10/Ara10.fragments.tsv.gz",
    
  •                  "D:/sn-CUT&TAG/2_fragments/ara.frag/ara11/Ara11.fragments.tsv.gz",
    
  •                  "D:/sn-CUT&TAG/2_fragments/ara.frag/ara12/Ara12.fragments.tsv.gz")
    

AraNames <- c("Ara9","Ara10","Ara11","Ara12")

ArrowFiles <- createArrowFiles(inputFiles = input.file.list,

  •                            sampleNames = AraNames,
    
  •                            minTSS = 1 ,
    
  •                            minFrags = 1000 ,
    
  •                            geneAnnotation = geneAnno,
    
  •                            genomeAnnotation = genomeAnno,
    
  •                            addTileMat = TRUE ,
    
  •                            TileMatParams = list(tileSize = 500, blacklist = NULL),
    
  •                            addGeneScoreMat = TRUE ,
    
  •                            GeneScoreMatParams = list(blacklist = NULL)
    
  • )
    Found Gene Seqnames not in GenomeAnnotation chromSizes, Removing : 1,2,3,4,5,Mt,Pt
    Found Exon Seqnames not in GenomeAnnotation chromSizes, Removing : 1,2,3,4,5,Mt,Pt
    Found TSS Seqnames not in GenomeAnnotation chromSizes, Removing : 1,2,3,4,5,Mt,Pt
    ArchR logging to : ArchRLogs\ArchR-createArrows-16c816882c00-Date-2025-03-31_Time-12-16-09.425782.log
    If there is an issue, please report to github with logFile!
    subThreading Enabled since ArchRLocking is FALSE see addArchRLocking
    2025-03-31 12:16:09.815 : Batch Execution w/ safelapply!, 0 mins elapsed.
    2025-03-31 12:16:09.823817 : (Ara10 : 1 of 4) Reading In Fragments from inputFiles (readMethod = tabix), 0 mins elapsed.
    2025-03-31 12:16:09.826849 : (Ara10 : 1 of 4) Tabix Bed To Temporary File, 0 mins elapsed.
    2025-03-31 12:19:16.903321 : (Ara10 : 1 of 4) Reading TabixFile 29 Percent, 3.118 mins elapsed.
    2025-03-31 12:22:19.778873 : (Ara10 : 1 of 4) Reading TabixFile 57 Percent, 6.166 mins elapsed.
    2025-03-31 12:24:46.916849 : (Ara10 : 1 of 4) Reading TabixFile 86 Percent, 8.618 mins elapsed.
    2025-03-31 12:24:47.610472 : (Ara10 : 1 of 4) Successful creation of Temporary File, 8.63 mins elapsed.
    2025-03-31 12:24:47.611335 : (Ara10 : 1 of 4) Creating ArrowFile From Temporary File, 8.63 mins elapsed.
    2025-03-31 12:26:31.167081 : (Ara10 : 1 of 4) Successful creation of Arrow File, 10.356 mins elapsed.
    2025-03-31 12:26:35.024639 : createArrowFiles has encountered an error, checking if any ArrowFiles completed..
    ArchR logging successful to : ArchRLogs\ArchR-createArrows-16c816882c00-Date-2025-03-31_Time-12-16-09.425782.log

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