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kallisto2nanosim
Simon Hegele edited this page May 26, 2025
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Converting transcript abundance files from Kallisto
for NanoSim.
Why is this conversion required?
- NanoSim can only read TSV-files with exactly three columns but Kallisto outputs five
- NanoSim has/had a bug where underscores in the target_id will cause it to fail
Examplary usecase:
For the evaluation of hybrid tools using short and long RNAseq reads you might want to simulate long reads with NanoSim that match the expression profile in your short read data.
usage: kallisto2nanosim [-h] [--remove_underscore] kallisto_file nanosim_file
Converting Kallisto transcript abundance files for NanoSim.
positional arguments:
kallisto_file
nanosim_file
options:
-h, --help show this help message and exit
--remove_underscore