You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
This part quantifies protein distributions in the final averaged and registered stacks.
To generate intensity plot profiles along the center-to-periphery and longitudinal basal body axis
we used Macro-RadialPlotProfile_single.ijm. It uses XY coordinates obtained at the end of the last part.
In the batch mode, it can be run using Macro-RadialPlotProfile_batch.ijm
and it needs to be provided with the a4_averaged/rescaled_avrg/registered folder as an input.
(in the provided dataset reordered output is located in Figure6/Input folder)
You would need to install ImageJ's "Radial Profile Angle" plugin for this part,
available at the original site or in our backup.
The (radial-cylindrical) intensity profiles are stored as csv files
and later maxima positions and FWHM are automatically quantified using maxima_quantification.ijm macro.