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Overview
github-actions[bot] edited this page Jul 21, 2026
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FLIMKit handles FLIM data from FLIM microscope systems and common TCSPC / time-tag formats (PicoQuant .ptu, Becker & Hickl .sdt, ISS time-tag, Photonscore .photons). It's designed as a replacement for FLIM microscope software, with two main workflows:
| Workflow | Description |
|---|---|
| Reconvolution fitting | Mono/bi/tri-exponential lifetime fitting with full IRF deconvolution, per-pixel and summed modes, multi-tile ROI stitching, and batch processing |
| Lifetime distribution fitting | Gaussian and Lorentzian continuous lifetime distributions (α(τ) models), per-ROI and per-pixel maps with GPU acceleration |
| Phasor analysis | Calibrated phasor plots with interactive elliptical cursors, automatic peak detection, two-component decomposition, and session save/load |
Both are accessible through a desktop GUI, guided terminal UI, CLI scripts, or the Python API.
Imaging files are reconstructed into a per-pixel decay cube (Y, X, H) from their scan / frame / line / pixel markers, and the intensity image is that cube summed over the time axis. Files without imaging markers (point, single-spot, FCS) are fit as a single decay with no image.