π DEG Pipeline & Visualizer β v3.4.0
Multi-Cancer Differential Gene Expression Analysis & Publication-Ready Visualization Suite
π About This Release
This release delivers a polished, production-ready build of DEG Pipeline & Visualizer β a desktop application that takes raw TCGA/GDC RNA-seq count data all the way through PyDESeq2-powered differential expression analysis to publication-quality Volcano, MA, Bar Chart, and Heatmap visualizations, entirely through a modern graphical interface.
β¨ What's New in v3.4.0
- 𧬠Refined DEG analysis engine built on PyDESeq2, with improved handling of GDC Sample Sheets and automatic sample-group normalization.
- π¨ Redesigned Scientific Dark theme alongside a clean Light theme, both optimized for long analysis sessions.
- π Four publication-ready plot types: Volcano Plot, MA Plot, Summary Bar Chart, and Expression Heatmap.
- π·οΈ Smarter gene labeling with automatic overlap avoidance via
adjustText, plus optional labeled/unlabeled output pairs. - πΌοΈ Live multi-plot preview with thumbnail navigation before committing to export.
- π€ Expanded export options β PNG, PDF, TIFF, and SVG at custom DPI, including a combined side-by-side composite figure.
- β‘ Background multi-threaded processing to keep the interface responsive during long-running analyses, with a live progress log.
- π Built-in guided tutorial and one-click citation generator, accessible directly from the About page.
- π©Ί Broad multi-cancer compatibility β works with any GDC/TCGA project (e.g. TCGA-COAD, TCGA-BRCA, TCGA-LUAD).
π» Downloads
Cross-platform support is provided for:
| Platform | Status |
|---|---|
| πͺ Windows | β Supported |
| π macOS | β Supported |
| π§ Linux | β Supported |
Download the build for your operating system from the assets attached to this release below, or run the application from source β see the installation guide in the project README.
π¦ Requirements
The complete, verified list of Python dependencies for this release is available in requirements.txt.
π How to Cite This Version
Balaei A. (2026). DEG Pipeline & Visualizer (v3.4.0) [Computer software].
https://github.com/alirezabk1382927-sys/DEG-Pipeline-Visualizer
π License Notice
Starting with this release, the project is distributed under a custom, restrictive, source-available proprietary license β replacing any previous MIT License. Copying, redistribution, or commercial use without explicit written permission from the Author is strictly prohibited. Full terms are available in LICENSE.
π Acknowledgements
Thank you to everyone using and supporting this project. If it helped your research, please β star the repository and cite the tool in your publications β it makes a real difference for continued development.
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