Exoglycosidases

alternativeTime edited this page Mar 24, 2014 · 2 revisions

Exoglycosidases Mode of Action

Commonly, used exoglycosidases for glycan structure characterisation

  • ABS: Arthrobacter ureafaciens sialidase releases α(2-6/3/8)-linked nonreducing terminal N-acetylneuraminic acid (NANA, Neu5Ac) and N-glycolylneuraminic acids (NGNA, Neu5Gc).
  • NAN1: Streptococcus pneumoniae sialidase releases α(2-3)-linked nonreducing terminal sialic acids (NANA and NGNA).
  • BTG: bovine testes β-galactosidase hydrolyzes nonreducing terminal galactose with β(1-3/4) linkages.
  • SPG: S. pneumoniae β-galactosidase hydrolyzes nonreducing terminal galactose β(1-4) linkages.
  • AMF: almond meal α-fucosidase releases α(1-3/4)-linked nonreducing terminal fucose residues except core α(1-6) fucose.
  • BKF: bovine kidney alpha-fucosidase releases α(1-2/6) fucose-linked nonreducing terminal fucose residues more efficiently than α(1-3/4)-linked fucose.
  • GUH: β-N-acetylglucosaminidase cloned from S. pneumoniae, expressed in Escherichia coli, will digest β(1-4)-linked GlcNAc to mannose but not a bisecting GlcNAc β(1-4) linked to mannose.
  • CBG: Jack Bean α(1-3,4,6)-Galactosidase Hydrolyses α(1-3,4,6)-linked terminal galactose residues.
  • JBM: Jack Bean α(1-2,3,6)-Mannosidase releases non-reducing terminal α(1-2,6)-linked mannose residues more efficiently than α(1-3).
Clone this wiki locally
You can’t perform that action at this time.
You signed in with another tab or window. Reload to refresh your session. You signed out in another tab or window. Reload to refresh your session.
Press h to open a hovercard with more details.