Data Analysis

Overview

Preparation

HEKA EPC10 Patchmaster voltage ramp sweeps need to be exported as .asc files (IV). Each file needs a corresponding entry in a summary .xlsx file, which contains the file name, the cell capacity and the number of the exported sweep. Different data sets can be combined in the summary .xlsx files sheets.

Internal Workflow

1. The median values of the currents at the holding potential are calculated.
2. Any unnecessary columns are removed from the .asc files and the data is cleaned up.
3. The ASC files and summary are linked manually.
4. The holding potential median values are written to the summary.
5. The offset is determined by subtracting the value at the reverse potential in the IVs from 0. This value is then written in the summary.
6. The current densities are calculated and written in the IVs and summary.
7. The current densities are offset-corrected and written in IVs and the summary.
8. The current densities inward/outward ratios are calculated and written in the summary.
9. The normalized slope conductance is calculated and written for IVs.
10. Outliers are identified using the iterative Grubbs method and are included in the summary.