What's New
PrimerCheck
Oligonucleotide analysis and primer design toolkit:
- Analyze — Tm (4 methods), MW, GC%, extinction coefficient, hairpin/self-dimer/hetero-dimer prediction, Tm mismatch heatmaps
- Check — In-silico PCR with mismatch tolerance
- Design — Primer3-powered primer design with full parameter control
- BLAST — NCBI BLAST search for specificity checking
Diversity
Combined alpha and beta diversity analysis:
- Alpha — 10 metrics (Observed Features, Chao1, ACE, Shannon, Simpson, Pielou's Evenness, Inverse Simpson, Berger-Parker, Faith's PD), rarefaction curves, statistical testing (Mann-Whitney, Kruskal-Wallis, Dunn's post-hoc)
- Beta — 7 distance metrics (Bray-Curtis, Jaccard, Canberra, UniFrac, Euclidean, Correlation, Aitchison), 4 ordination methods (PCoA, NMDS, t-SNE, PCA), statistical tests (PERMANOVA, PERMDISP, ANOSIM, pairwise PERMANOVA, Mantel)
SeqConvert
- Added light/dark theme toggle matching other tools
Docker
docker pull eriklysoe/biotools:v1.0.2