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Jane Ling edited this page Oct 31, 2023 · 5 revisions

This project aims to extract multi-cellular spontaneous synaptic activities from a recording of iGluSNFR3.

With the assumption that the synaptic events would result in a faster increase in fluorescence intensity than noise and drifts, the method of slope detection and thresholding is used for spike detection.

Functionality Highlights

  1. Effectively picking up synaptic events from noise image

  2. Effectively identifying the spatial-peak temporal-peak of each event, hence separating synapses close to each other image

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