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Added a compact napari trial timeline rail and viewer HUD that follow the movie frame slider using photodiode-aligned stimulus epoch windows.
Added GUI-independent grid and watershed ROI extraction helpers with optional region-mask restriction for script workflows.
Added response-watershed ROI extraction that segments repeatable stimulus-locked pixel responses using amplitude, local response coherence, and split-half reliability score maps, including selection from the napari ROIs tab.
Added a dF/F no_baseline_epoch baseline mode for recordings without a distinct baseline epoch, exposed in the napari Plot tab as no baseline epoch with a First epoch selector.
Added parity-only psycho5 grid and watershed-label helpers for comparing native twopy ROI discovery against historical MATLAB ROI extraction.
Added a tracked dated CSV-backed pixel-size calibration resolver and micron-sized grid ROI helper for calibrated physical ROI templates.
Added ROIs-tab ROI mode controls in napari with manual drawing as the default plus generated grid, watershed, and response-watershed ROI modes.
Added metadata-driven calibration profile preselection for ROIs-tab micron grids, backed by tracked ScanImage config mappings and conservative manual fallback when the profile is incomplete or unmeasured.
Changes
Rendered gray, grey, and interleave epochs as neutral gray in the napari trial timeline.
Simplified ROIs-tab grid controls so pixel and micron settings show only for the selected unit, extrapolation is enabled by default, calibration modes include known ScanImage config names, and micron-grid status reports only the estimated pixel size.
Made response-watershed ROI masks split disconnected score islands, fill component holes by default, and expose an opt-in closing radius for tiny same-basin gaps.
Renamed the napari Update tab to Metadata, grouped Metadata into Recording, Microscope, and Outputs sections, made Search Database and Load manually the first Load-tab actions, moved Reload saved analysis to Load, moved Save ROIs + analysis to Export, and removed the Recompute preview button.
Fixes
Kept unresolved ROIs-tab calibration fields on explicit selection placeholders instead of silently using the first dropdown option.
Made script and napari dF/F defaults use the same gray/grey/interleave baseline epoch selection.
Made napari live ROI plot updates more responsive by cancelling stale background recomputes, reusing unchanged ROI traces, reusing epoch plot widgets, and caching Labels colormap bases for visibility toggles.
Made napari correlation filtering uncheck and dim ROIs that do not pass QC in the ROIs tab and Labels layer.
Made Reload saved analysis restore the saved ROI file into the editable Labels layer.