Research preprint v1.3.0

Research preprint v1.3.0

Adds the fixed one-update fresh-expression pulse study (059) to the existing private-memory paper. Its primary terminal population-accuracy contrast is unresolved: +0.037 [-0.247, +0.336] percentage points relative to unchanged historical retrieval. The interval also overlaps the separate one-expected-matching-bit benchmark. This is not evidence of equivalence or absence of effect.

Restoring historical retrieval after the common first update improves terminal founder representation relative to continued fresh expression: +2.995 [1.465, 4.688] points. This is a secondary ancestry outcome, not demonstrated population benefit or superiority over unchanged historical retrieval. Negative first-update ancestry, frequent founder loss, two continuous-F survivors, unresolved comparisons and exact first-update coupling identities remain in the record. New transfer inputs reuse all 192 selected 057 preparations; no independent training cohort is added.

Includes revised manuscript/supplement, 16 figures, all 15,203 saved-table estimates, scientific source through 059, seed provenance, compact time series/fates/adverse outcomes and scoped audits. Raw candidate trajectories and random tapes remain outside the compact release. No new simulations are needed for its saved-table and figure checks.

This is a revision of the same paper. Earlier tags and DOI versions are preserved. Manuscript/data: CC BY 4.0; original code: MIT. AI assistance is disclosed; no external peer review is claimed.