v0.pre
Pre-release
Pre-release
This is the first release of scSplit as a genotype-free tool for demultiplexing pooled single cell samples.
This version of scSplit toolset contains below script:
sc_split_matrices.py: build SNV x barcode Matrices (reference and alternative alleles)
sc_split_main.py: main script to conduct reference free AF-based demultiplexing
sc_split_vcf.py: generate vcf file containing simulated genotypes according to demultiplexing assignments
sc_split_simu.py: simulate BAM and allele fraction matrices based on provided vcf and BAM reads