Spheroid Analysis v1.0.0

Spheroid Analysis v1.0.0

A user-friendly desktop application for analyzing microscopy images of spheroids.

Features

• Three-Region Analysis: Automatically identifies background, border, and core regions
• Intensity-Based Core Detection: Uses original grayscale values to find the darkest region
• Adjustable Parameters: Adjust parameters manually in the UI
• Crop/Select Region: Restrict analysis to specific image regions
• Export Results: Saves annotated images and statistics

Installation

macOS

1. Download SpheroidAnalysis-macOS.zip
2. Extract the ZIP file
3. Right-click SpheroidAnalysis.app → Open
4. If prompted, click "Open" to allow the unsigned app

Windows

1. Download SpheroidAnalysis-Windows.zip
2. Extract the ZIP file
3. Double-click SpheroidAnalysis.exe
4. If Windows Defender warns, click "More info" → "Run anyway"

Linux (Debian/Ubuntu)

1. Download SpheroidAnalysis-Linux-Debian.zip
2. Extract the ZIP file
3. Open terminal in extracted folder
4. Run: chmod +x SpheroidAnalysis && ./SpheroidAnalysis

Linux (RedHat/CentOS/Fedora)

1. Download SpheroidAnalysis-Linux-RedHat.zip
2. Extract the ZIP file
3. Open terminal in extracted folder
4. Run: chmod +x SpheroidAnalysis-redhat && ./SpheroidAnalysis-redhat

System Requirements

• Windows: 64-bit Windows 7 or later
• macOS: macOS 10.13 (High Sierra) or later
• Linux: 64-bit Debian 11+, Ubuntu 20.04+, or RHEL 9+

Known Issues

• First launch may be slow (5-10 seconds)
• Multiple attempts may be required on the first launch (unknown developer issue)

Changelog

• Initial release
• Core detection based on grayscale intensity
• Connected component filtering
• Percentage-based crop/select feature
• Border detection with edge sensitivity feature
• Compact UI