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mcianfrocco edited this page Dec 16, 2014 · 16 revisions
A wiki protocol that will help replicate image processing results from Cianfrocco & Leschziner, 2014.

Table of contents:

  1. [Download micrographs and data] (https://github.com/mcianfrocco/Cianfrocco-and-Leschziner-2014-EMCloudProcessing/wiki/Download-micrographs-and-data)
  2. [Extract particles] (https://github.com/mcianfrocco/Cianfrocco-and-Leschziner-2014-EMCloudProcessing/wiki/Extract-particles)
  3. [Perform 3D classification] (https://github.com/mcianfrocco/Cianfrocco-and-Leschziner-2014-EMCloudProcessing/wiki/Perform-3D-classification)
  4. [Refine single model to high resolution] (https://github.com/mcianfrocco/Cianfrocco-and-Leschziner-2014-EMCloudProcessing/wiki/Refine-single-model-to-high-resolution)
  5. [Calculate local resolution & filter map] (https://github.com/mcianfrocco/Cianfrocco-and-Leschziner-2014-EMCloudProcessing/wiki/Calculate-local-resolution-and-filter-map)
  6. [Visualize final structure using Chimera (colored by local resolution)] (https://github.com/mcianfrocco/Cianfrocco-and-Leschziner-2014-EMCloudProcessing/wiki/Visualize-final-structure-using-Chimera-(colored-by-local-resolution))

Table of contents:

[Home] (https://github.com/mcianfrocco/Cianfrocco-and-Leschziner-2014-EMCloudProcessing/wiki)

Spot instances:

  1. [Retrieve spot instance price histories] (https://github.com/mcianfrocco/Cianfrocco-and-Leschziner-EMCloudProcessing/wiki/Retrieve-spot-instance-price-histories)
  2. [Calculate percentage time] (https://github.com/mcianfrocco/Cianfrocco-and-Leschziner-EMCloudProcessing/wiki/Calculate-percentage-time-spot-instance-prices-spend-below-given-price)

Cryo-EM:

  1. [Download micrographs and data] (https://github.com/mcianfrocco/Cianfrocco-and-Leschziner-2014-EMCloudProcessing/wiki/Download-micrographs-and-data)
  2. [Extract particles] (https://github.com/mcianfrocco/Cianfrocco-and-Leschziner-2014-EMCloudProcessing/wiki/Extract-particles)
  3. [Perform 3D classification] (https://github.com/mcianfrocco/Cianfrocco-and-Leschziner-2014-EMCloudProcessing/wiki/Perform-3D-classification)
  4. [Refine single model to high resolution] (https://github.com/mcianfrocco/Cianfrocco-and-Leschziner-2014-EMCloudProcessing/wiki/Refine-single-model-to-high-resolution)
  5. [Calculate gold standard FSC & filter map] (https://github.com/mcianfrocco/Cianfrocco-and-Leschziner-2014-EMCloudProcessing/wiki/Calculate-gold-standard-FSC-&-filter-model)

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