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v4.2.0
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Key Updates
Output File Handling:
The --tsv flag has been deprecated. TSV output is now the default format.
If input is name fp rp, adding support for other output formats.
For both mfeprimer and mfeprimer spec, results will be saved as out.spec.tsv.
Additional outputs for mfeprimer:
Dimer analysis: out.dimer.tsv;
Hairpin analysis: out.hairpin.tsv.
Bug Fixes:
Resolved issue #26 ;
Mismatch not working in tsv mode;
Sub-command Enhancements:
New sub-commands are now available:
mfeprimer search: homology search for short sequences like primers against genome database, require pre-indexed by mfeprimer index;
mfeprimer degen: convert a DNA sequence from ambiguous (with degenerate bases) to unambiguous DNA list;
mfeprimer split-fasta: split fasta into N parts with almost equal size;
mfeprimer thermo: thermodynamics calculation for oligos.
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