relative quantification with delta Cq and delta delta Cq
Pre-release
Pre-release
Upgraded features for relative quantification, new vignettes to explain these, and gave functions more helpful names such as calculate... as well as create...
- lay out and display 96/384-well plates for easy experimental setup (
label_plate_rowcol,create_blank_plate, ...) - read-in Cq and raw data from Roche LightCycler machines with single-channel fluorescence (
read_lightcycler_1colour_cq,read_lightcycler_1colour_raw) - delta Cq: normalization/ relative quantification of Cq data to one or more reference targets by delta count method (
calculate_normcq,calculate_deltacq_bysampleid) - delta delta Cq: normalization of delta Cq data across multiple samples (
calculate_deltadeltacq_bytargetid) - calibration of primer sets including estimating efficiencies and visualization of curves (
calculate_efficiency, and see vignettes) - visualization of amplification and melt curves (
calculate_drdt_plate, and see vignettes)