Enhancements & fixes
- Added an option to control the version ("revision") of the blobDir.
Use when rerunning the pipeline on an assembly. - Restructured locations and names of output files, see output.md
- The
samplesamplesheet column can now contain/to organise theread_mapping/outputs in sub-directories. - Added parameter
--window_sizeto partition sequences and calculate per-window statistics - Updated
blobtk/imagestoblobtk/plot(an nf-core module), and patched the module for version0.8.1which is not yet available via bioconda. - Added the
gridplot generation fromblobtk/plot. - Updated
blast/blastndb path to be staged as aBLASTN_DB_DIRdue to an edge case where:could cause a malformed path. - Versions are now output via topic where possible (e.g. where tools are run prior to version collection).
Parameters
| Old parameter | New parameter |
|---|---|
| --revision | |
| --window_size |
Software dependencies
Note, since the pipeline is using Nextflow DSL2, each process will be run with its own Biocontainer. This means that on occasion it is entirely possible for the pipeline to be using different versions of the same tool. However, the overall software dependency changes compared to the last release have been listed below for reference. Only Docker or Singularity containers are supported, conda is not supported.
| Dependency | Old version | New version |
|---|---|---|
| blast | 2.15.0 | 2.17.0 |
| blobtk | 0.5.1 | 0.8.1 |
| diamond | 2.1.16 | 2.1.23 |
| gunzip | 1.1 | 1.13 |
| minimap2 | 2.28 | 2.29 |
| samtools | 1.20 and 1.21 | 1.21 and 1.23.1 |
NB: Dependency has been updated if both old and new version information is present.
NB: Dependency has been added if just the new version information is present.
NB: Dependency has been removed if version information isn't present.