UserGuide

DropBot v3 User Guide

DropBot DB3-120 kit

You should have received the following items with your DropBot kit:

1. DropBot DB3-120 instrument
2. +12V power supply
3. Test board
4. USB A/B cable
5. 3x DMF chips
6. Device spacer
7. Webcam
8. Webcam holder
9. Test tube containing 0.1 M NaCl + 0.1% Tetronic 90-R4

Install MicroDrop

MicroDrop is a Windows app for programming and controlling the DropBot and the latest release can be downloaded here.

MicroDrop currently supports Windows 7/8/10.

Note: If you are running Windows 7, you will also need to install this driver before connecting the DropBot.

Connect DropBot

1. Plug +12V power supply into wall outlet.
2. Connect +12V power supply to DropBot.
3. Connect USB cable between PC and DropBot.

Run the on-board hardware diagnostic tests

Once you have MicroDrop installed and have connected the DropBot, the next thing you will want to do is test that your hardware arrived in a working state:

1. Launch MicroDrop from the Windows Start menu.
2. From the Tools\DropBot menu, select Run all on-board self-tests.

Note: If you have any trouble connecting to the DropBot, please see the Troubleshooting guide.

The software will pop up a wizard showing you how to insert the test board into your DropBot. These tests confirm that:

1. The high-voltage source is working.
2. The feedback-sensing circuit is working.
3. There are no short-circuits on any of the actuation channels.
4. All actuation channels are functional.

See below for an example report:

Attach the Webcam

Attach the webcam to the DropBot as shown below:

Note: For (optional) advanced control over webcam settings (e.g., fixed focus), download the Logitech Webcam Software from the Logitech C920 support page.

Insert a DMF chip

The following steps are illustrated in the figure below (see here for a larger image):

1. Pick up DMF chip by edges, being careful not to touch the surface.
2. Insert DMF chip into front of DropBot, and;
3. ...push forward until it hits the back of the chip guide.
4. Insert device spacer under the chip guide surface.
5. Push device spacer in as far as possible.

Load liquid onto the chip

To load liquid on the DMF chip:

1. Actuate a reservoir electrode using the MicroDrop software.
   
2. Manually pipette ~6 uL of liquid at edge of top plate over reservoir electrode.
   

DMF chip specifications and sample mask

• DropBot v3 pogo-pin mapping
• SCI-BOTS 90-pin array v3 device mask: svg (for loading in MicroDrop), dwg