``` R dat <- data.frame(x = 1:10,y = 1:10,grp = rep(letters[1:2],length.out = 10)) ggplot(dat,aes(x = x,y = y)) + geom_point(aes(colour = grp)) + scale_colour_manual(values = c('#FF00001A','#0000FF1A')) ``` reported by Joran.